Review



luclite plus reporter gene assay  (Revvity)


Bioz Verified Symbol Revvity is a verified supplier
Bioz Manufacturer Symbol Revvity manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 93
      Buy from Supplier

    Structured Review

    Revvity luclite plus reporter gene assay
    Effect of NPM1 Ser-125 phosphorylation on HIV-1 transcription . A and B , effect of expression of NPM1 and its phosphorylation mutants (S125A and S125D) on basal (panel A ) and Tat-mediated HIV-1 transcription (panel B ). HEK293T cells were transfected with vectors expressing the indicated GFP-tagged NPM1 mutants and cotransfected with vectors expressing HIV-1 LTR-luciferase reporter (panel A ) or HIV-1 LTR-luciferase reporter and FLAG-tagged Tat expression vector (panel B ). Luciferase activity was analyzed using <t>Luclite</t> plus Reporter Gene Assay and normalized to GFP intensity. All data were shown in triplicates as transcription activation fold related to the WT NPM1. C , effect of NPM1 Ser-125 phosphorylation on the interaction with Tat protein. GFP-tagged NPM1 (WT, S125A, and S125D) and Flag-tagged HIV-1 Tat were expressed in 293T cells. Tat was precipitated with anti-Flag antibody, resolved on SDS-PAGE, and immunoblotted with anti-GFP to detect NPM1 and anti-Flag antibodies to detect Tat. Lane 1, IgG control; Lane 2, No Tat; Lane 3, Tat+NPM1 WT; Lane 4, Tat+NPM1 S125A; Lane 5, Tat+NPM1 S125D. D – G , quantification of the coimmunoprecipitation data from three independent experiments. NPM1’s 62 kDa and 250 kDa isoforms were normalized to NPM1 and Tat input, respectively. Asterisks indicate p < 0.05 (∗) and p < 0.01 (∗∗). HIV-1, human immunodeficiency virus-1; LTR, long terminal repeat; NPM1, nucleophosmin.
    Luclite Plus Reporter Gene Assay, supplied by Revvity, used in various techniques. Bioz Stars score: 93/100, based on 155 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/luclite plus reporter gene assay/product/Revvity
    Average 93 stars, based on 155 article reviews
    luclite plus reporter gene assay - by Bioz Stars, 2026-03
    93/100 stars

    Images

    1) Product Images from "HIV-1 Transcription Inhibitor 1E7-03 Decreases Nucleophosmin Phosphorylation"

    Article Title: HIV-1 Transcription Inhibitor 1E7-03 Decreases Nucleophosmin Phosphorylation

    Journal: Molecular & Cellular Proteomics : MCP

    doi: 10.1016/j.mcpro.2022.100488

    Effect of NPM1 Ser-125 phosphorylation on HIV-1 transcription . A and B , effect of expression of NPM1 and its phosphorylation mutants (S125A and S125D) on basal (panel A ) and Tat-mediated HIV-1 transcription (panel B ). HEK293T cells were transfected with vectors expressing the indicated GFP-tagged NPM1 mutants and cotransfected with vectors expressing HIV-1 LTR-luciferase reporter (panel A ) or HIV-1 LTR-luciferase reporter and FLAG-tagged Tat expression vector (panel B ). Luciferase activity was analyzed using Luclite plus Reporter Gene Assay and normalized to GFP intensity. All data were shown in triplicates as transcription activation fold related to the WT NPM1. C , effect of NPM1 Ser-125 phosphorylation on the interaction with Tat protein. GFP-tagged NPM1 (WT, S125A, and S125D) and Flag-tagged HIV-1 Tat were expressed in 293T cells. Tat was precipitated with anti-Flag antibody, resolved on SDS-PAGE, and immunoblotted with anti-GFP to detect NPM1 and anti-Flag antibodies to detect Tat. Lane 1, IgG control; Lane 2, No Tat; Lane 3, Tat+NPM1 WT; Lane 4, Tat+NPM1 S125A; Lane 5, Tat+NPM1 S125D. D – G , quantification of the coimmunoprecipitation data from three independent experiments. NPM1’s 62 kDa and 250 kDa isoforms were normalized to NPM1 and Tat input, respectively. Asterisks indicate p < 0.05 (∗) and p < 0.01 (∗∗). HIV-1, human immunodeficiency virus-1; LTR, long terminal repeat; NPM1, nucleophosmin.
    Figure Legend Snippet: Effect of NPM1 Ser-125 phosphorylation on HIV-1 transcription . A and B , effect of expression of NPM1 and its phosphorylation mutants (S125A and S125D) on basal (panel A ) and Tat-mediated HIV-1 transcription (panel B ). HEK293T cells were transfected with vectors expressing the indicated GFP-tagged NPM1 mutants and cotransfected with vectors expressing HIV-1 LTR-luciferase reporter (panel A ) or HIV-1 LTR-luciferase reporter and FLAG-tagged Tat expression vector (panel B ). Luciferase activity was analyzed using Luclite plus Reporter Gene Assay and normalized to GFP intensity. All data were shown in triplicates as transcription activation fold related to the WT NPM1. C , effect of NPM1 Ser-125 phosphorylation on the interaction with Tat protein. GFP-tagged NPM1 (WT, S125A, and S125D) and Flag-tagged HIV-1 Tat were expressed in 293T cells. Tat was precipitated with anti-Flag antibody, resolved on SDS-PAGE, and immunoblotted with anti-GFP to detect NPM1 and anti-Flag antibodies to detect Tat. Lane 1, IgG control; Lane 2, No Tat; Lane 3, Tat+NPM1 WT; Lane 4, Tat+NPM1 S125A; Lane 5, Tat+NPM1 S125D. D – G , quantification of the coimmunoprecipitation data from three independent experiments. NPM1’s 62 kDa and 250 kDa isoforms were normalized to NPM1 and Tat input, respectively. Asterisks indicate p < 0.05 (∗) and p < 0.01 (∗∗). HIV-1, human immunodeficiency virus-1; LTR, long terminal repeat; NPM1, nucleophosmin.

    Techniques Used: Expressing, Transfection, Luciferase, Plasmid Preparation, Activity Assay, Reporter Gene Assay, Activation Assay, SDS Page, Control, Virus



    Similar Products

    luclite plus reporter gene assay  (Revvity)
    93
    Revvity luclite plus reporter gene assay
    Effect of NPM1 Ser-125 phosphorylation on HIV-1 transcription . A and B , effect of expression of NPM1 and its phosphorylation mutants (S125A and S125D) on basal (panel A ) and Tat-mediated HIV-1 transcription (panel B ). HEK293T cells were transfected with vectors expressing the indicated GFP-tagged NPM1 mutants and cotransfected with vectors expressing HIV-1 LTR-luciferase reporter (panel A ) or HIV-1 LTR-luciferase reporter and FLAG-tagged Tat expression vector (panel B ). Luciferase activity was analyzed using <t>Luclite</t> plus Reporter Gene Assay and normalized to GFP intensity. All data were shown in triplicates as transcription activation fold related to the WT NPM1. C , effect of NPM1 Ser-125 phosphorylation on the interaction with Tat protein. GFP-tagged NPM1 (WT, S125A, and S125D) and Flag-tagged HIV-1 Tat were expressed in 293T cells. Tat was precipitated with anti-Flag antibody, resolved on SDS-PAGE, and immunoblotted with anti-GFP to detect NPM1 and anti-Flag antibodies to detect Tat. Lane 1, IgG control; Lane 2, No Tat; Lane 3, Tat+NPM1 WT; Lane 4, Tat+NPM1 S125A; Lane 5, Tat+NPM1 S125D. D – G , quantification of the coimmunoprecipitation data from three independent experiments. NPM1’s 62 kDa and 250 kDa isoforms were normalized to NPM1 and Tat input, respectively. Asterisks indicate p < 0.05 (∗) and p < 0.01 (∗∗). HIV-1, human immunodeficiency virus-1; LTR, long terminal repeat; NPM1, nucleophosmin.
    Luclite Plus Reporter Gene Assay, supplied by Revvity, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/luclite plus reporter gene assay/product/Revvity
    Average 93 stars, based on 1 article reviews
    luclite plus reporter gene assay - by Bioz Stars, 2026-03
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    Effect of NPM1 Ser-125 phosphorylation on HIV-1 transcription . A and B , effect of expression of NPM1 and its phosphorylation mutants (S125A and S125D) on basal (panel A ) and Tat-mediated HIV-1 transcription (panel B ). HEK293T cells were transfected with vectors expressing the indicated GFP-tagged NPM1 mutants and cotransfected with vectors expressing HIV-1 LTR-luciferase reporter (panel A ) or HIV-1 LTR-luciferase reporter and FLAG-tagged Tat expression vector (panel B ). Luciferase activity was analyzed using Luclite plus Reporter Gene Assay and normalized to GFP intensity. All data were shown in triplicates as transcription activation fold related to the WT NPM1. C , effect of NPM1 Ser-125 phosphorylation on the interaction with Tat protein. GFP-tagged NPM1 (WT, S125A, and S125D) and Flag-tagged HIV-1 Tat were expressed in 293T cells. Tat was precipitated with anti-Flag antibody, resolved on SDS-PAGE, and immunoblotted with anti-GFP to detect NPM1 and anti-Flag antibodies to detect Tat. Lane 1, IgG control; Lane 2, No Tat; Lane 3, Tat+NPM1 WT; Lane 4, Tat+NPM1 S125A; Lane 5, Tat+NPM1 S125D. D – G , quantification of the coimmunoprecipitation data from three independent experiments. NPM1’s 62 kDa and 250 kDa isoforms were normalized to NPM1 and Tat input, respectively. Asterisks indicate p < 0.05 (∗) and p < 0.01 (∗∗). HIV-1, human immunodeficiency virus-1; LTR, long terminal repeat; NPM1, nucleophosmin.

    Journal: Molecular & Cellular Proteomics : MCP

    Article Title: HIV-1 Transcription Inhibitor 1E7-03 Decreases Nucleophosmin Phosphorylation

    doi: 10.1016/j.mcpro.2022.100488

    Figure Lengend Snippet: Effect of NPM1 Ser-125 phosphorylation on HIV-1 transcription . A and B , effect of expression of NPM1 and its phosphorylation mutants (S125A and S125D) on basal (panel A ) and Tat-mediated HIV-1 transcription (panel B ). HEK293T cells were transfected with vectors expressing the indicated GFP-tagged NPM1 mutants and cotransfected with vectors expressing HIV-1 LTR-luciferase reporter (panel A ) or HIV-1 LTR-luciferase reporter and FLAG-tagged Tat expression vector (panel B ). Luciferase activity was analyzed using Luclite plus Reporter Gene Assay and normalized to GFP intensity. All data were shown in triplicates as transcription activation fold related to the WT NPM1. C , effect of NPM1 Ser-125 phosphorylation on the interaction with Tat protein. GFP-tagged NPM1 (WT, S125A, and S125D) and Flag-tagged HIV-1 Tat were expressed in 293T cells. Tat was precipitated with anti-Flag antibody, resolved on SDS-PAGE, and immunoblotted with anti-GFP to detect NPM1 and anti-Flag antibodies to detect Tat. Lane 1, IgG control; Lane 2, No Tat; Lane 3, Tat+NPM1 WT; Lane 4, Tat+NPM1 S125A; Lane 5, Tat+NPM1 S125D. D – G , quantification of the coimmunoprecipitation data from three independent experiments. NPM1’s 62 kDa and 250 kDa isoforms were normalized to NPM1 and Tat input, respectively. Asterisks indicate p < 0.05 (∗) and p < 0.01 (∗∗). HIV-1, human immunodeficiency virus-1; LTR, long terminal repeat; NPM1, nucleophosmin.

    Article Snippet: Cells were cultured 48 h post-transfection, and luciferase activity was analyzed using Luclite plus Reporter Gene Assay (PerkinElmer) measured by Glo-Max Microplate Multimode reader (Promega).

    Techniques: Expressing, Transfection, Luciferase, Plasmid Preparation, Activity Assay, Reporter Gene Assay, Activation Assay, SDS Page, Control, Virus